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. 2014 Jun 9;9(6):e96914. doi: 10.1371/journal.pone.0096914

Figure 3. Sequence alignment of ginsenoside-transforming BglPC28 and Bgp1 or structure-determined glycoside hydrolase family 3 enzymes.

Figure 3

The sequence alignment was created using ClustalW at the EBI-server using default settings and visualized with Jalview (http://www.ebi.ac.uk/Vmichele/jalview/). Regions of identity or high similarity among sequences are shown as black or gray columns, respectively. The conserved active site residues (general acid/base and nucleophile residues) were marked by asterisk. The PA14 domains of S.ven and K.mar are boxed. Genbank IDs of the glycoside hydrolases family 3 are as follows, Pseudonocardia sp. Gsoil 1536 β-glucosidase [BglPC28 (This study)], JX960416; Microbacterium esteraromaticum KACC 16318 β-glucosidase (Bgp1), AEX88466; Streptomyces venezuelae β-glycosidase (S.ven), AAC68679; ThermotoganeapolitanaDSM 4359T β-glycosidase (T.nea), ABI29899; Kluyveromycesmarxianus β-glucosidase (K.mar), ACY95404.