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. 2014 Jun 9;9(6):e96914. doi: 10.1371/journal.pone.0096914

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© 2014 Du et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lane: 1, uninduced crude extract; 2, soluble fraction of the crude extract of the induced recombinant BL21 (DE3) cells; 3, precipitated fraction of the crude extract of the induced recombinant BL21 (DE3) cells; 4, GST-BglPC28 after purification with the GST•bind agarose resin; 5, purified recombinant BglPC28 after cleavage via thrombin.