Table 1. Effects of metal ions and reagents on the activity of purified recombinant BglPC28.
Metal ions or regents | Relative activity ± SD (%) at: | |
1 mM | 10 mM | |
NaCl | 106.0±6.02 | 126.7±3.82 |
KCl | 109.2±2.22 | 121.7±5.68 |
MgCl2 | 111.0±8.74 | 121.9±0.91 |
MnCl2 | 112.4±2.01 | 104.5±8.89 |
CoCl2 | 130.0±7.37 | 139.5±2.57 |
ZnCl2 | 110.1±2.69 | 124.4±4.59 |
CaCl2 | 111.3±1.71 | 123.3±3.13 |
CuCl2 | 103.3±6.46 | 108.4±7.71 |
HgCl2 | 1.1±0.534 | 2.0±0.74 |
SDS | 0.9±0.540 | 1.1±0.81 |
EDTA | 98.2±0.73 | 92.6±0.66 |
β-Mercaptoethanol | 94.5±7.50 | 94.4±5.22 |
DTT | 92.4±3.41 | 90.5±4.24 |
Control | 100±5.02 | 100±6.03 |