Table 2. Relative activity of purified recombinant BglPC28 towards various chromogenic substrates as measured by PNP or PNP release at 37°C. ND: not determined.

Substratea		Relative activity ± SD (%)b
1	pNP-α-D-glucopyranoside	7.6±2.4
2	pNP-α-D-mannopyranoside	ND
3	pNP-α-D-xylopyranoside	ND
4	pNP-α-L-arabinofuranoside	ND
5	pNP-α-L-arabinopyranoside	ND
6	pNP-α-L-rhamnopyranoside	ND
7	pNP-β-D-fucopyranoside	ND
8	pNP-β-D-galactopyranoside	ND
9	pNP-β-D-glucopyranoside	100.0±1.6
10	pNP-β-D-glucosaminide	7.3±5.5
11	pNP-β-D-mannopyranoside	ND
12	pNP-β-D-xylopyranoside	11.0±2.7
13	pNP-β-L-arabinopyranoside	14.5±3.6
14	oNP-α-D-galactopyranoside	ND
15	oNP-β-D-fucopyranoside	ND
16	oNP-β-D-galactopyranoside	ND
17	oNP-β-D-glucopyranoside	ND
18	pNP-α-L-D-fucopyranoside	ND

^aFinal concentration, 2.0 mM.

^bActivity toward pNP-β-D-glucopyranoside was set as 100%.