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. 2014 Jun 9;9(6):e98900. doi: 10.1371/journal.pone.0098900

Figure 6. Syntaxin 11 recruits Munc18-2 to cytoplasmic membranes and to the activating immunological synapse.

Figure 6

Analysis of the localization of Munc18-2 in YTS NK cells. (A) YTS cells were transfected with mCherry-Munc18-2, stained with LysoTracker Green to visualize secretory lysosomes and either imaged immediately (Resting) or conjugated with 721.221 target cells pre-stained with Cell Trace Far Red (blue in the merge image panels). (B) YTS cells were co-transfected with mCherry-Munc18-2 and GFP-syntaxin 11 and either imaged alone or after incubation with 721.221 cells pre-stained with Cell Trace Far Red. Cells were imaged using a Zeiss LSM700 laser scanning confocal microscope. Scale bars 5 µm.