Table 2. Peptides used in SipA binding assays.
Protein | Peptide name | Sequence |
Cpa signal-peptide | Cpa | FSIRAFGAEEQ(K) FLUR |
FctA signal-peptide | FctA | MSQNVKAEGGV(K) FLUR |
Mutant FctA signal-peptide | FctA_mut | MSQNLKREGGV(K) FLUR |
FctA N-terminal mature peptide | FctA_cut | EGGVSTGSILN(K) FLUR |
FctB signal-peptide | FctB | FNQTVLAKDST(K) FLUR |
FctA sorting peptide | FctA_QVPTG | FLUR QVPTGVVGTLAP |
FLUR = Carboxyfluorescein; ala-X-ala motif highlighted in bold text; sorting motif in bold italics.