Figure 7. Cell lineage construction and associated cell behaviors after offline editing.

(A) Cell trajectories for all tracked cells in the-field-of-view. The color of trajectories indicates cell generations. Blue, green, red, cyan, and magenta represent trajectories from the first to the fifth generations, respectively. (B) Cell trajectories for cells presented in the first video frame and their offspring cells. (C) Trajectories for cells from a selected cell lineage family. (D) Cell lineage families constructed with automated cell tracking program followed by offline editing. (E) A selected cell lineage family with cell migration speed, cell area, and cell axis ratio aligned with cell cycle progression. Note that a maximum of 5 generations and 11 cell divisions in total are observed in this family during 40 hrs of monitoring. (F) The enlarged figure for the selected area in figure (E).