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Total DNA (≥4 µg) of each sample was firstly digested with EcoR V and BamH I. The probe was hybridized with the bar gene. The size of hybridized fragment should be 600 bp. Lane 1, pPSCB; lane 2, PSCB1; Lane 3, PSCB2; lane 4, wild-type of P. subcordiformis.
