Table 3. Primers and restriction sites of the fragments for the chloroplast vectors.

fragments	Primers	Sequences	Enzymic sites	Length
rrn16–trnI	16-I for	cggggtaccTCCTACGGGAGGCAGCAGT	KpnI	1288 bp
	16-I rev	gcgtcgacTTGAGGCAAATGGGCTATGC	SalI
trnA–rrn23	A-23 for	ttgcggccgcACAACGGAGTTTCGGAATA	NotI	1405 bp
	A-23 rev	cgagctcCGTTACTCAAACCGACATTC	SacI
atpA 5′ UTR	5′atpA for	gcgtcgacAAGCTTATCGATGACTTTATTAG	SalI	668 bp
	5′atpA rev	ccgatatcGGACATTTTCACTTCTGGAGT	EcoRV
rbcL 3′ UTR	3′rbcL for	cgggatccGTACTCAAGCTCGTAACGAAG	BamHI	448 bp
	3′rbcL rev	gctctagaGGATCGCACTCTACCGATT	XbaI
the bar gene	bar for	ccgatatcATGAGCCCAGAACGACGCC	EcoRV	567 bp
	bar rev	cgggattcTCATCAAATCTCGGTGACGG	BamHI
the gfp gene	GFP for	ccgatatcGTACTCAAGCTCGTAACGAAG	EcoRV	732 bp
	GFP rev	cgggattcGGATCGCACTCTACCGATT	BamHI