Figure 3.

FACs analysis of the apoptosis and cell cycle progression of OVCAR-3 cells. (A) PZH was not found to induce apoptosis in the OVCAR-3 cells collected and stained with Annexin V/PI. Annexin V-positive/PI-negative stained cells (lower right) and Annexin V/PI double-positive stained cells (upper right) represent early and late apoptosis, while Annexin V-negative and PI-positive stained cells (upper left) represent dead cells. Data are presented as the mean ± standard deviation from three independent experiments ^*P<0.05 vs. control cells. (B) PZH blocked G₁/S progression in the OVCAR-3 cells that were collected and stained with Annexin V/PI and analyzed by FACS. Following treatment with consecutive concentrations of PZH (0, 250, 500 and 1,000 μg/ml), the number of cells in the G₁ phase increased by 50.99, 61.23, 74.76 and 91.81%, respectively (P=0.004), while the percentage of cells in S-phase was 30.98, 32.11, 25.243 and 8.19%, respectively (P=0.022). PZH, Pien Tze Huang; FACS, fluorescence-activated cell sorting; PI, propidium iodide.