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. 2014 Mar 14;7(6):2023–2028. doi: 10.3892/ol.2014.1972

Figure 1.

Figure 1

CD133+ SFCs derived from the MHCC97 cell line possess characteristics of LCSCs. (A) CD133+ cell subpopulation, sorted from the hepatocellular carcinoma MHCC97 cell line by magnetic activated cell sorting, overexpressed the stem cell surface marker, CD133, detected by flow cytometry using PE-conjugated anti-human CD133 antibody. #P<0.05 compared with the CD133− cell group. *P<0.05 compared with the PCs(B and C) CD133+ cells derived from the MHCC97 cells and PCs formed liver cancer spheroids in stem cell-conditioned medium (magnification, ×100). Data are expressed as the mean ± standard deviation (n=3). *P<0.05 compared with the PCs. (D) The sphere formation of single cells in six-well plates was detected on the first, third, sixth (magnification, ×400) and ninth day (magnification, ×40). (E) Hematoxylin and eosin staining revealed histological characteristics in tumor xenografts derived from CD133+ SFCs comparable with the PCs (magnification, ×100). CD133, cluster of differentiation 133; SFCs, sphere-forming cells; LCSCs, liver cancer stem cells; PE, R-phycoerythrin; PC, parental cell; d, days.