Table I.

Primer sequences used in the polymerase chain reaction for detecting the three marker genes.

Gene	Primer sequence	Annealing temperature, °C	Product, bp
FAM83A			318
Outer		72
Forward	5′-CGCCACTGTGTACTTCCAGACCGTCAAGC-3′
Reverse	5′-CCTCGGCGGTTCTGCTCATGCTCCACTC-3′
Inner		60
Forward	5′-GTGGGGTGTTCGTTTGTG-3′
Reverse	5′-GCTTGGAGGAGGCGTAG-3′
NPY1R			372
Outer		72
Forward	5′-GCGTTCCAAAATGTAACACTTGATGCGTACA-3′
Reverse	5′-CATCTGTGTGCATCGTGGACATGGCTATTGT-3′
Inner		60
Forward	5′-CACTCTTCTCTTGGTGCTG-3′
Reverse	5′-GTTTTTGTTCAGGAACCCA-3′
KRT19			393
Outer		72
Forward	5′-AAGCTAACCATGCAGAACCTCAACGACCGC-3′
Reverse	5′-TTTTATTGGCAGGTCAGGAGAAGAGCC-3′
Inner		60
Forward	5′-CAGCCACTACTACACGACC-3′
Reverse	5′-ACCTCATATTGGCTTCGCA-3′
GAPDH		60	452
Forward	5′-ACCACAGTCCATGCCATC-3′
Reverse	5′-TCCACCACCCTGTTGCTGTA-3′