Table II.
Identification of the mRNA expression level of the OCT1 gene in cervical cancer and adjacent non-cancerous tissues by qPCR.
| CT, means ± standard deviation | ||||||
|---|---|---|---|---|---|---|
|
|
||||||
| Sample | n | GAPDH | OCT1 | Δ | ΔΔ | Folda |
| Cervical cancer | 10 | 16.56±1.32 | 27.47±1.51 | 10.91±0.84 | −2.58±0.63 | 5.98 |
| Non-cancerous tissues | 10 | 16.23±1.25 | 29.72±1.67 | 13.49±0.92 | ||
a
Mean fold change in expression of the target gene, OCT1, relative to the internal control gene, GAPDH, was calculated using the 2−ΔΔCT equation previously adopted by Livak et al (10): ΔΔCT = (CTTarget - CTGAPDH)cervical cancer - (CTTarget - CTGAPDH)control. At least three replicates of each reaction were performed.
CT, threshold cycle; qPCR, quantitative polymerase chain reaction; OCT1, octamer transcription factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.