Figure 1.

Concentration-dependent specific lysis of MCF7 cells by TGFα-PE38. MCF7 cells were incubated with increasing concentrations of TGFα-PE38 for 24, 48 or 72 h. The percentage of specific lysis (%SL) was determined by the XTT assay. This data is representative of two experiments using the XTT assay. Bars are standard error of the mean (N=3). Error bars are hidden in some of the data points. Other assays, including the Trypan Blue Cell Viability Solution assay, the DePsiphler mitochondrial potential assay and the in situ chromium uptake assay, produced similar results, but with a wider range of error. TGF, tumor growth factor; PE, Pseudomonas endotoxin.