Figure 2.

Time-dependent specific lysis of MCF7 cells by TGFα-PE38. MCF7 cells were incubated with 5 ng/ml TGFα-PE38 for 1, 2 or 3 days. The percentage of specific lysis (%SL) was determined by the XTT assay. This data is representative of two experiments using the XTT assay. Bars are standard error of the mean (n=3). Other assays, including the trypan blue viability assay and the in situ chromium uptake assay, produced similar results, but with a wider range of error. TGF, tumor growth factor; PE, Pseudomonas endotoxin.