Skip to main content
. 2014 Mar 12;7(6):2113–2117. doi: 10.3892/ol.2014.1969

Figure 6.

Figure 6

Specific lysis of MCF7 by CTLs, preincubated (PreCTLs) or not (CTL) with TGFα-PE38 (T-PE), and CTLs co-incubated with T-PE (CTL + T-PE) or not. MCF7 cells were incubated with CTLs preincubated with 0.25 ng/ml T-PE for one day (PreCTL) or not (CTL) at an effector (CTL)-to-target (MCF7) ratio (E:T) of 1.25 for one day. The third group was CTLs at an E:T of 1.25 co-incubated with or without 0.25 ng/ml T-PE for one day (CTL + T-PE). The percentage of specific lysis (%SL) was determined by the XTT assay. Preincubation of CTLs with T-PE (Pre CTL) vs. CTL, P=0.48 (NS; Mann-Whitney Rank Sum test). CTLs co-incubated with T-PE (CTL + T-PE) vs. CTL, *P=0.01. Pre CTL vs. CTL + T-PE, P=0.22 (NS). Results were similar for E:T ratios of 0.625 and 2.5. Bars are standard error of the mean (n=3). TGF, tumor growth factor; PE, Pseudomonas endotoxin; CTL, cytotoxic T lymphocytes; NS, not significant.