Figure 4. CM-118 and its combination with afatinib induce apoptosis in H1993 cells. (A) H1993 plated in 6-well culture plates were treated for 48 h with 2 μmol/L CM-118, 2 μmol/L afatinib, or a combination of the two inhibitors, then assessed for viability by trypan-blue cell counting. Percent net growth or death is relative to the initial cell density at initiation of treatment. (B) Cells as in (A) were immunoblotted. H1993 cells infected with the indicated lantivirus shRNAs were similarly treated and subjected to analysis of immunoblotting (C) and cell survival (D). H1993 cells transiently transfected with the indicated expression constructs were similarly analyzed by immunoblotting (E) and assayed for survival (F). Statistical analysis was performed on cells of Sh-Bim vs. Sh NT; cells with Mcl-1 overexpression vs. vector-transfected cells.