Figure 3.KRAS insertion mutants promoted anchorage-independent growth in soft agar. NIH3T3 cells stably transfected with pcDNA3.1 empty vector (EV), wild-type KRAS (WT), G12V KRAS mutant (G12V), ¹⁰G¹¹ and ¹¹GA¹² mutants were cultured in soft agar for analysis. Representative microscopic pictures of colony from each transfectant were taken (Magnification, 400×). The number of colony in each transfectant was plot in the bar chart and the results shown were mean and standard deviation from three independent experiments. The P value of < 0.05 and < 0.001 were denoted as * and ** respectively.