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. 2014 Jun 9;9(6):e99656. doi: 10.1371/journal.pone.0099656

Figure 5. Woven bone content is decreased at later stages of fracture healing in HFD-fed mice.

Figure 5

(A) Representative Alcian Blue Hematoxylin/Orange G stained histologic sections of fracture callus in lean- and HFD-fed mice at 7, 10, 14, 21, 28, and 35 days post-fracture. Quantification of cartilage (B), woven bone (C), and stromal cell areas (D), expressed as percentages of total callus area, were determined using a point-counting histomorphometric method. Bars represent means ± SEM (n≥5). ***p<0.001 compared to time-matched controls using two-way, unpaired ANOVA with Bonferroni post-tests. The black size marker in the lower right panel  = 1 mm.