Table 1. Primers used for amplification and quantification of Helicoverpa armigera cDNA.
Gene | Primers | Sequence (from 5′ to 3′) | Length | T°m | Amplicon size |
EF-1α | EF-1α-F EF-1α-R | GACAAACGTACCATCGAGAAG GATACCAGCCTCGAACTCAC | 21 20 | 62 | 279 |
CYP6AE14 | CYP6AE14-F CYP6AE14-R | TGTGCATTTGGCGTTGAA TCCGAGATGTGGGCGTAT | 18 18 | 54 | 241 |
CYP6B2 | CYP6B2-F CYP6B2-R | CCTGAAAGATTCTTCGCGGAGGAATTGGACAGCAGCTTCGTGATGC | 24 22 | 70 | 140 |
CYP9A12 | CYP9A12-F CYP9A12-R | ATCACCTCATAGAAGATATCC CATGTCTTTCCATTCTTGACC | 21 21 | 59 | 233 |