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. 2014 Jun 9;9(6):e99229. doi: 10.1371/journal.pone.0099229

Table 1. Primers used for amplification and quantification of Helicoverpa armigera cDNA.

Gene Primers Sequence (from 5′ to 3′) Length T°m Amplicon size
EF-1α EF-1α-F EF-1α-R GACAAACGTACCATCGAGAAG GATACCAGCCTCGAACTCAC 21 20 62 279
CYP6AE14 CYP6AE14-F CYP6AE14-R TGTGCATTTGGCGTTGAA TCCGAGATGTGGGCGTAT 18 18 54 241
CYP6B2 CYP6B2-F CYP6B2-R CCTGAAAGATTCTTCGCGGAGGAATTGGACAGCAGCTTCGTGATGC 24 22 70 140
CYP9A12 CYP9A12-F CYP9A12-R ATCACCTCATAGAAGATATCC CATGTCTTTCCATTCTTGACC 21 21 59 233