Table 1. Sequences of primers and probes used for Real-Time PCR. Sequences related to gene type X collagen and MMP13 are proprietary to Applied Biosystems Inc. and not disclosed.
Gene | Forward Primer | Reverse Primer | Probe |
GAPDH | AGGGCTGCTTTTAACTCTGGTAAA | GAATTTGCCATGGGTGGAAT | CCTCAACTACATGGTTTAC |
COL II | GGCAATAGCAGGTTCACGTACA | CGATAACAGTCTTGCCCCACTT | CTGCACGAAACATAC |
Aggrecan | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGTGTAGAGA | ATGGAACACGATGCCTTTCACCACGA |