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. 2014 Jun 9;9(6):e99587. doi: 10.1371/journal.pone.0099587

Table 1. Sequences of primers and probes used for Real-Time PCR. Sequences related to gene type X collagen and MMP13 are proprietary to Applied Biosystems Inc. and not disclosed.

Gene Forward Primer Reverse Primer Probe
GAPDH AGGGCTGCTTTTAACTCTGGTAAA GAATTTGCCATGGGTGGAAT CCTCAACTACATGGTTTAC
COL II GGCAATAGCAGGTTCACGTACA CGATAACAGTCTTGCCCCACTT CTGCACGAAACATAC
Aggrecan TCGAGGACAGCGAGGCC TCGAGGGTGTAGCGTGTAGAGA ATGGAACACGATGCCTTTCACCACGA