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. 2014 Jun 9;9(6):e99575. doi: 10.1371/journal.pone.0099575

Figure 1. DT treatment efficiently depletes macrophages.

Figure 1

For microscopic images of macrophages, macrophages were collected from peritoneal lavage of LysMCre/iDTR mice (A) 2 days after DT treatment (10 ng/g body weight) and (H) 7 days after stopping DT injection. Macrophages were collected from (B) peritoneal lavage or (C) bone-marrow of LysMCre/iDTR mice, then treated with saline or DT (0.25 ng/ml) for 8 hours. mRNA levels of macrophage marker F4/80 and CD68 were measured in (D) WAT, (E) liver, and (F) skeletal muscle of mice treated with saline or DT for 2 days. mRNA levels of M2 macrophage marker ARG1 were measured in (G) WAT of mice treated with saline or DT for 2 days and 7 days after stopping DT injection. Data are represented as mean ± SEM. Asterisks denote significant differences *P<0.05, **P<0.01 vs. saline-treated mice.