Figure 2. CXCR7+ EC display altered morphology and lack contact inhibition. pLEC were infected with Trans+CXCR7. At 20 h post-infection cultures were fixed and stained by IFA for PECAM-1 (green), CXCR7 (red), and DAPI. (A) A field of interest was selected and a z-stack series of 40 images with 0.1 µM step size was photographed at 100X magnification. The image was subjected to deconvolution analysis. Nuclei of CXCR7 negative cells are marked with white stars, the nucleus of the CXCR7+ cell is marked with a white triangle. Scale bar is 20 µm. A region of interest (white box) containing both CXCR7 staining and junctional CD31 staining was extracted and (B) subjected to 3-dimensional analysis. White dashed lines denote the region in the x-y plane containing junctional CD31 staining.