Figure 6. CXCR7 expression enhances EC invasion toward CXCR7 ligands. iLEC were infected with Trans or Trans+CXCR7. At 20 h post-infection cells were labeled with Calcein-AM dye, trypsinized, and transferred to matrigel-coated Fluoroblok™ invasion plates with ITAC/CXCL11 at 50 ng/ml, SDF-1/CXCL12 at 50 ng/ml, or no ligand in the bottom chamber. Invasion was measured via fluorescence accumulation in the bottom chamber at the indicated timepoints. Individual wells were normalized to fluorescence intensity at t = 0 and RFU increases were averaged for each condition. n = 16 wells per condition and data are representative of three independent experiments. **P < 0.001 for both CXCR7+SDF-1 and CXCR7+ITAC compared with unstimulated Trans control. A subset of cells was stained prior to seeding for HA-CXCR7 by flow cytometry to control for adenovirus transduction efficiency (histogram, inset).