Figure 1.S. pombe nucleoporins fused to GFP. (A) Subcellular localization of GFP-tagged nucleoporins. The different images were obtained with the same acquisition times and processed in parallel. Deconvolved images are shown. The top left two panels show fluorescence (left) and bright field (right) images of an spCut11-GFP expressing cell. Nucleoporins were classified into seven groups according to localization in the NPC inferred from localization of the budding yeast orthologs. The scale bar represents 10 μm. (B-D) Localization of GFP-spSec13 (B), spAmo1-GFP (C), and spTts1-GFP (D) in wild type and cells lacking spNup132 (nup132Δ); in (B), GFP was fused with N-terminus (left) and C-terminus (right) of spSec13. spCut11-mCherry was simultaneously observed as a known nucleoporin marker. Yellow arrows indicate NPC-clustering regions in nup132Δ cells (lower panels). The scale bars represent 10 μm.