Figure 2. Quantification of GFP-tagged nucleoporins. (A) Fluorescence intensities of GFP-tagged nucleoporins. Fluorescence intensities of about 50 cells from each strain cultured at 26 °C were measured. Average values after background subtraction are shown in the bar graph. Error bars represent standard deviations. Components of the Nup107-Nup160 subcomplex are shown in magenta. (B) Quantitative western blot analysis of Nup107-Nup160 subcomplex nucleoporins. GFP-fused nucleoporins were fractioned by SDS-PAGE and detected by anti-GFP antibody (left). The membranes were stripped and reprobed to detect endogenous spNup189n as an internal control (right). The numbers above the images indicate cell numbers used to prepare the whole cell extract for each lane. (C) The relative amounts of Nup107-Nup160 subcomplex nucleoporins based on quantitative western blot analysis. Protein band intensities for 1 × 10⁶ cells in (B) were measured for quantification. See Materials and Methods for detail.