Table 4. Function of non-essential nucleoporins for various growth conditions.
| genotypes | 26 °C | 30 °C | 36 °C | 20 μg/ml TBZ | 10 mM HU |
|---|---|---|---|---|---|
| wild type | ++++ | ++++ | ++++ | +++ | +++ |
| ∆pom34 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆pom152 | ++++ | ++++ | ++++ | +++S | +++ |
| ∆ely5 | ++++ | ++++ | ++++ | ++ | + |
| ∆nup37 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆nup120 | +++S | ++SS | - | - | - |
| ∆seh1 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆nup131 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆nup132 | ++++ | ++++ | ++++ | +SS | +S |
| ∆nup131 ∆nup132 | ++++ | ++++ | ++++ | +SSS | +S |
| ∆nup40 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆nup184 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆npp106 | ++++ | ++++S | +++SS | + | +++ |
| ∆nup60 | ++++ | ++++ | ++++ | +++ | ++SS |
| ∆nup61 | ++++ | ++++ | ++++ | +++ | +++ |
| ∆nup124 | ++++ | ++++ | ++++ | ++SS | ++SS |
| ∆amo1 | ++++S | +++S | +++S | +++ | +++ |
| ∆mph1 | ++++ | ++++ | ++++ | - | ++S |
| ∆cds1 | ++++ | ++++ | ++++ | +++ | - |
Each cell strain was cultured to midlog phase in liquid YES. 5-fold serial dilutions of each transformant were spotted onto YES plates with or without HU or TBZ. Viable colony number is indicated by -, +, ++, +++, and ++++: “-” indicates no colony formation. Colony size (growth rate) is indicated by S, for small; SS, for smaller; SSS, for smallest colonies. Δmph1 and Δcds1strains were used as positive controls sensitive to TBZ and HU, respectively. For ∆nup120 strains, TBZ sensitivity and HU sensitivity were assayed at 26 °C.