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. 2014 Feb 17;13(8):1277–1287. doi: 10.4161/cc.28190

Figure 2.

Figure 2.

E2F1 impairs ATRA-induced osteogenic differentiation of U2OS cells. (A and B) Overexpression of E2F1 inhibits ATRA-induced osteogenic differentiation. The protein and mRNA levels of OPN were determined (A), and the activities of ALP were evaluated (B). (C and D) Silencing of E2F1 promotes ATRA-induced osteogenic differentiation. The levels of OPN were determined (C), and the activities of ALP were evaluated (D). (A–D) U2OS cells transfected with E2F1 plasmid (A and B) or E2F1 siRNA (C and D) along with corresponding control were treated with 1 μM ATRA for 7 d. *, P < 0.05.