Figure 4. STAT3/IL-17F expression is driven by an IL-15-independent pathway. (A and B) Malignant (MF2059) T cells were transiently transfected with small interfering RNA against IL-15 or non-target control (NT) and cultured for 24 h. After incubation, RNA was purified from the cells and reverse transcribed to cDNA and analyzed by quantitative PCR to determine the relative level of IL15, IL17F, and GAPDH mRNA. In each sample, the level of IL15 mRNA or IL17F mRNA was normalized to the amount of GAPDH mRNA and depicted as fold change when compared NT control. There was a significant difference in IL-15 expression (P value = 0,002). (C) Malignant (MF2059) T cells were transiently transfected with small interfering RNA against IL-15 or non-target control (NT). Twenty-four hours after transfection, cells were washed and cultured for another 24 or 48 h. The cells were lysed and the lysates analyzed by western blotting using antibodies against pY-STAT3, STAT3, and actin.