Figure 4. Hybrid binucleated cells with DNA damages enter and complete mitosis. (A) Representative images and (B) percentage of γH2AX-positive mitotic hybrid binucleated cells from 3T3 H2B-EGFP cells fused with HCT116 H2B–mCherry cells. Green, mouse genome; red, human genome; blue, γH2AX; Type I, γH2AX foci on HCT116 chromosomes only; Type II, γH2AX foci on both 3T3 and HCT116 chromosomes. (C) Representative images of hybrid daughter cells in interphase stained for γH2AX. (D) Statistical results. Bars = 20 μm. ***P < 0.001, 2-tailed t test. Mean ± SD, from 3 independent experiments.