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. 2014 Jun 9;9(6):e99542. doi: 10.1371/journal.pone.0099542

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© 2014 Peng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

293T-BAC36, 293T-BACΔ6 cells were transfected with ori-Lyt plasmids (pOri-A) plus others as indicated (PCR3.1-ORF50 and FLAG-ORF6). KSHV lytic replication was induced by the expression of ORF50. Total DNAs were isolated from the transfected cells. Replicated DNAs were distinguished from input DNAs by DpnI digestion and detected by southern blotting with Dig-labeled pOri-A probe.