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. 2014 Jun 9;9(6):e99481. doi: 10.1371/journal.pone.0099481

Figure 3. Human melanoma cells MV3 express NOX4, but not NOX5 or NOX2-related NADPH oxidase subunits.

Figure 3

Total RNAs were extracted from MV3 melanoma cells and from human polymorphonuclear neutrophils (A–B, PMN), human melanocytes (B, NGM) or from a human microvasculature endothelial cells linage (C, HMEC-1), used as positive controls. The expression levels of p47phox (A), NOX4 (B) or NOX5 (C) were analyzed by RT-PCR, using GAPDH expression as internal control as described in Materials and Methods. (D) NOX4, NOX2, p22phox, p40phox, p47phox, p67phox and βTubulin protein expression were detected by western blotting as described in Materials and Methods. Data are expressed as mean ± SD of three independent experiments and images are representative of three independent experiments with similar results.