Table 2.

Cytopathic effect (CPE) observed in E-11 cell monolayers infected with RGNNV, SJNNV, RGNNV/SJNNV, SJNNV/RGNNV betanodavirus strains at different incubation temperatures

	15 °C				20 °C				25 °C				30 °C
	RGNNV	SJNNV	RGNNV/SJNNV	SJNNV/RGNNV	RGNNV	SJNNV	RGNNV/SJNNV	SJNNV/RGNNV	RGNNV	SJNNV	RGNNV/SJNNV	SJNNV/RGNNV	RGNNV	SJNNV	RGNNV/SJNNV	SJNNV/RGNNV
T1	§	§	§	§	-	-	-	-	-	-	-	-	-	-	-	-
T3	§	§	§	§	-	-	-	-	-	-	-	-	+ + +	-	+ + +	-
T5	§	§	§	§	+	+	+	+	+ +	+ +	+ +	+ +	+ + + +	-	+ + + +	+
T7	*	*	*	*	+ + + +	+ + + +	+ + + +	+ + + +	+ + + + +	+ + + + +	+ + + + +	+ + + + +	+ + + + +	+ +	+ + + + +	+ +
T8	*	*	*	*	+ + + + +	+ + + + +	+ + + + +	+ + + + +						+ + +		+ + +
T9	*	*	*	*										+ + + +		+ + + +
T10	*	*	*	*										+ + + +		+ + + +

Data were recorded at T₁ (20 hpi), T₃ (45 hpi), T₅ (69 hpi), T₇ (93 hpi), T₈ (117 hpi), T₉ (141 hpi) and T₁₀ (165 hpi). Cells appearance, estimation of the CPE and its degree of severity were assessed according to the following levels: cell shrinkage (§); alteration of the cell monolayer characterized by dark and contracted cells with the tendency to detach from the surface of the flask (*); absence of CPE (-); cellular alteration and presence of diffuse vacuoles (+); presence of diffuse foci of rounded, granular and vacuolated cells (+ +); presence of diffuse foci of rounded, granular and vacuolated cells and initial cell monolayer disruption (+ + +); presence of diffuse foci of rounded, granular and vacuolated cells and advanced cell monolayer disruption (+ + + +); complete cell monolayer disruption (+ + + + +).