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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Tissue Eng Regen Med. 2013 Jan 14;9(11):1277–1285. doi: 10.1002/term.1645

Figure 3. Characterization of ASC and vascular graft creation within the rabbit model.

Figure 3

(A) RTPCR analysis of eNOS mRNA expression rabbit ASC differentiated towards and EC lineage before (control, C) and after (Ad-eNOS) eNOS transfection. (B) Phase contrast photomicrograph of rabbit ASC differentiated towards an EC lineage after application of shear stress (1.5 dyne/cm2 at 0.2 Pa, 48h) demonstrating alignment of the cells in the direction of shear. (C) Phase contrast photomicrograph of EC-differentiated rabbit ASC following seeding onto Matrigel demonstrating cord formation indicative of angiogenic potential. (D) Laser confocal micrograph of the luminal surface of a vascular scaffold seeded with differentiated and transfected autologous rabbit ASC and flow conditioned for 5d demonstrates the adherence and alignment of the seeded cells (Cell Tracker Green).