Figure 1. STAT1 is involved in IDO expression in response to T lymphocyte-derived factors.

MDA-231 were transfected with siRNA against STAT1 or scrambled siRNA before activation with IFN-γ or supernatants of cultured TIL for 30 minutes (pSTAT1) or 24 h. Protein extracts were prepared for STAT1 (phosphorylated and total), IDO and β-actin immunoblot analysis. Results are representative of three independent experiments.