Figure 5.ISM1 expression in the adult mouse. (A) RT-PCR for full-length ISM1 in different tissues of the adult (3-mo-old) mouse. GADPH is used as an internal control. The highest levels of ISM1 mRNA are observed in lung and brain tissues. Lower ISM1 expression levels are found in bone marrow, heart, kidney, ovary, and testis. (B–M) Immunohistochemistry for ISM1 in adjacent sections of the heart (B–E), kidney (F–I), and liver (J–M) of the adult mouse counterstained with hematoxylin. Pre-absorption of the anti-ISM1 antibody with a BP reveals no staining in the heart (B and D), kidney (F and H), and liver (J and L). ISM1-positive cells are found in the myocardium of the heart ventricle (C and E), renal tubules of cortex of the kidney (G and I), and hepatocytes of the liver (K and M). ctx, cortex; CV, central vein; gl, glomerulus; hep, hepatocyte; he vent, heart ventricle; RTu, renal tubules. Scale bar = 200 μm in (F and G); scale bar = 100 μm in (B, C, J, and K); scale bar = 50 μm in (D, E, H, I, L, and M).