Figure 3. Role of TRRAP in B-cell proliferation and cell cycle progression. (A) Negatively (CD43⁻) sorted B cells were stained with CFSE and induced to switch with LPS+IL4, and their proliferation ability tracked by FACS for the indicated days (n = 3). (B) Resting B cells were induced to switch with LPS+IL4 for 3 d. Aliquots were labeled on a daily basis with PI and cell distribution at the cell cycle phases analyzed by FACS using ModFit software. The bottom panels are a compilation of the data shown in the top panels; n = 2 with 3 mice of each genotype in each experiment.