Fig. 2.

Pull down assays for protein–protein interactions: Hexa Histidine tag based pull down assays were carried out to demonstrate protein-protein interactions in vitro. (a) SDS PAGE analysis of samples stained with commassie blue Lanes 1–3 shows pull down of 6x His tagged ΔN70 Pro, VPg, and p8 after incubation with untagged RdRp respectively. (b) Western blot analysis of respective samples from (a) with anti RdRp antibodies. (c) Coexpression of 6x His-p10 and untagged RdRp followed by pull down of 6x His tag p10 with Ni–NTA beads. Lane 1, p10 alone; lane 2 6x His-p10 coexpressed with RdRp which does not contain His tag. (d) Western blot analysis of samples from (c) with anti RdRp antibodies.