Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Coexpression and pull down assay of untagged RdRp C-terminal deletion mutants with 6xHis-p10: Untagged RdRp C-terminal deletion mutants were coexpressed with 6xHis-tagged p10 and pull down was carried out using Ni–NTA beads. (a) SDS–PAGE analysis of eluted proteins after pull down; lane 1 shows pull down of His-p10 alone; lanes 2 & 3 show pull down of RdRp CΔ43 and RdRp CΔ85 with His-p10 respectively. (b) Western blot analysis of respective samples from (a) with anti RdRp antibodies.
