Supplementary Fig. 2.

Protein expression analysis: (a) Coexpression of RdRp, RdRpCΔ43 and RdRpCΔ85 (lacks His tag) with p10 (contains N-terminal His tag). The corresponding RdRp and p10 positions were shown by arrow. (b) Western blot analysis of respective samples from (a), western blotting was carried out first with anti His tag antibody followed by same blot re-probed with RdRp antibody. Empty vector (pRSF duet) transformed cells were used as control (c) Expression and SDS–PAGE analysis of pET22b RdRp and RdRpCΔ43. Empty vector (pET22b) transformed cells were used as control.