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Differential nucleolar RNA qPCR analysis protocol. (A) Scheme of nucleolar denaturation and RT-PCR protocol. (B) Small RNA qPCR controls of denatured and non-denatured nucleoli. Bars represent mean (N = 3 biological experiments) and error bars SD. (C) qPCR for heat-denatured and non-denatured nucleoli for the indicated small RNAs and miRNAs. ΔCt represents Ct denaturated nucleoli – Ct non-denatured nucleoli.
