Fig. 5.

Relative abundance of miRNAs in the subcellular compartments as analyzed by deep sequencing. (A) Comparison of miRNA reads between the nucleus and cytoplasm. MiRNAs with reads ⩾10 in the nucleus, cytoplasm and total cellular fractions were identified (N = 127) and nuclear and cytoplasmic reads were normalized against the total number of cellular reads. The nuclear/cytoplasmic read ratio is shown. Insets display miRNAs in the highest and lowest 15% ratio. (B) Relative frequencies of nucleolar reads as compared to nuclear (gray bar) and cytoplasmic (black bars) reads are shown.