Fig. 6.

Effect of CRM1 and Pol I transcription inhibition on nucleolar miRNA abundance. Cells were treated with ActD (50 ng/ml) (A) or leptomycin B (LMB) (10 μM) (B) for 3 h, fractionated and RNA was isolated. RNA (25 μg) was separated on 15% gel and hybridized to respective LNA probes. Relative proportion of miRNAs is expressed as compared to total cellular RNA set as 1. (C) Quantitative analysis of (B) for miR-31 and hY1. Results represent the mean and SEM of two biological experiments.