Table 1.
Percentage of surviving CD9+ GC-B cells in the presence or absence of HK cells.
| Day of culture | CD9+ GC-B cells (%) |
|||||
|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp. 3 | Exp. 4 | Exp. 5 | ||
| Day 0 | 58.5 | 26.2 | 48.5 | |||
| Day 1 | −HK | 30.5 | 39.9 | 21.4 | 26.1 | 58.7 |
| +HK | 59.0 | 54.2 | 41.0 | 33.5 | 71.1 | |
| +HK (transwell) | 27.3 | 22.8 | 57.8 | |||
GC-B cells were cultured with CD40L in the presence or absence of HK cells for 24 h. In some experiments, GC-B cells are separated from HK cells by transwell inserts. At the end of the culture, cells were stained with anti-CD9 or isotype control antibody and analyzed by flow cytometry. Percentage of the surviving CD9+ GC-B cells was determined.