Figure 4.

Colocalization pattern of P2Y₁₂ (in green) with Iba1 and ED-1 (in red). (A) Upregulation of P2Y₁₂ expression in ipsilateral brain section surrounding the ischemic core (dotted line). Scale bar=1 mm. (B) P2Y₁₂ is constitutively coexpressed on Iba1⁺ glial cells in intact side (see high magnification for ramified microglia; scale bar=10 μm). P2Y₁₂ expression is increased in the ramified activated microglia at the outer border of the ischemic lesion (asterisk in A). In contrast, P2Y₁₂ is downregulated in amoeboid microglia present in the core of the ischemic lesion (pound sign in A). Scale bar=50 μm. (C) P2Y₁₂ receptor expression was observed only on few ED-1⁺ cells. Scale bar=50 μm.