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. 2014 Mar 26;34(6):1047–1059. doi: 10.1038/jcbfm.2014.54

Figure 3.

Figure 3

Real-time PCR validation of differentially expressed enzymes. Data confirmed that compared with Tat.AG, Tat.B upregulates zinc endopeptidases matrix metalloproteinase (MMP)-3 (A), MMP-10 (B), MMP-12 (C), the enzymes transglutaminase-2 (TGM2) (D), and chitinase-3-like protein 2 (CHI3L2) (E) in human brain microvascular endothelial cells (HBMEC). Controls are untreated HBMEC; Tat.AG represents HBMEC treated with Tat.AG; Tat.B represents HBMEC treated with Tat.B; Tat.AG-HI represents HBMEC treated with heat-inactivated Tat.AG. All Tat proteins were used at 100 ng/mL, and all cells were treated for 48 hours. Data using HBMEC from three different human donors are shown, and each donor was tested in triplicate. (***P<0.001, compared with cells treated with Tat.AG, cells treated with heat-inactivated Tat, or untreated controls). Full names of genes are provided in Table 1.