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. 2014 May 28;5:3915. doi: 10.1038/ncomms4915

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(a) Schematic of the experimental protocol used for the analysis of proliferation (top) and neuron survival (bottom) in 6-month-old control, A30P α-SYN and α-SYN KO mice. Black arrows indicate time points of BrdU-injection while grey arrowheads show time points of perfusion, followed by immunohistochemistry. (b) Immunofluorescent images of neuronal precursor cells (BrdU, green) in the SVZ in control, A30P α-SYN and α-SYN KO mice: z-stack projections of confocal series. Scale bar, 10 μm. (c) Summary plots of the number of BrdU-labelled cells mm⁻³ SVZ in all three groups (n=3 mice per group; P=0.894 for control/A30P α-SYN; P=0.919 for control/α-SYN KO; P=0.998 for A30P α-SYN/α-SYN KO). (d) Immunofluorescent micrographs of mature, ~32-day-old adult-born (BrdU, green) neurons (NeuN, magenta) in the OB GC layer in control, A30P α-SYN and α-SYN KO mice: z-stack projections of confocal series. White arrows point to soma of double-labelled (adult-born) GCs (merge). Scale bar, 10 μm. (e) Summary plots showing the percentage of BrdU/NeuN-labelled neurons in the GC layer of control, A30P α-SYN and α-SYN KO mice (n=5, 6 and 6 mice per group, respectively; P=0.0081 for control/A30P α-SYN; P=0.148 for control/α-SYN KO; P=0.260 for A30P α-SYN/α-SYN KO). (f) Immunofluorescent images of immature, ~21-day-old adult-born (BrdU, green) neurons (NeuN, magenta) in the GC layer in control and A30P α-SYN mice: z-stack projections of confocal series. White arrows point to soma of double-labelled (adult-born) GCs (merge). Scale bar, 10 μm. (g) Summary plots demonstrating the percentage of BrdU/NeuN-labelled neurons in the GC layer of both groups (n=5 mice per group; P=0.0097). (h) Immunofluorescent images of immature (DCX, red) adult-born GCs in sections counterstained with DAPI (blue) in control and A30P α-SYN mice: z-stack projections of confocal series. White arrows point to cell bodies of some immature GCs. Scale bar, 10 μm. (i) Summary plots of the numbers of DCX-positive GCs mm⁻³ in both groups (n=6 mice per group; P=0.0263). Values are presented as mean±s.e.m. One-way analysis of variance/Tukey–Kramer post hoc test (c,e); Student’s t-test (g,i).