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. 2014 May 30;5:4000. doi: 10.1038/ncomms5000

Figure 8. Time course of formation of Brachyury-positive mesodermal middle layer.

Figure 8

As described in Fig. 7a, A single OGTR1 ESC was grown in 3D 90-Pa fibrin gel in +LIF condition for the first 11 h before switching to –LIF condition. After 60 h, the individual colony is transferred to the top of collagen-1-coated polyacrylamide gel in the absence of LIF. The Brachyury-positive cells were observed to localize or segregate at one end of the colony before sorting into a distinct middle mesodermal layer after 84 h. About 50% of the cells (out of 50 single ESCs) from >3 separate experiments that were tracked from time zero to 120 h showed similar results. Scale bar, 50 μm.