Figure 1.

GHR Is Expressed in the Human Mammary Gland Epithelium

(A–C) Immunostaining of normal breast epithelium shows that GHR is expressed mostly in clusters and rarely as scattered cells. Representative images of eight samples.

(D–F) Double IF of normal breast epithelium shows that GHR is expressed mostly in ALDH1A1+ cells or in their immediate proximity.

(G and H) Single IF for GHR and ALDH1A1 in consecutive normal breast sections.

(I) Detail of (E), showing ALDH1A1+/GHR+ cells.

(J–L) Double IF for ALDH1A3 and GHR shows isolated GHR+ cells adjacent to ALDH1A3+ cells (arrowheads). Representative images of five samples.

(M) Flow cytometry for GHR and CD10 showing no overlap between cells expressing these markers. Representative data from two different samples.

(N) Flow cytometry for GHR and CK18 showing a small overlap (4%) between these markers. Representative data from two different samples.